May 7, 2016 1 Comment
Half a month ago, I introduced you to automated time-lapse microscopy, but I showed the analysis of only a single static image. I didn’t take advantage of the rich time-series that the microscope provides for us. A richness that becomes clearest with video:
Above, you can see two types of non-small cell lung cancer cells growing in the presence of 512 nmol of Alectinib. The cells fluorescing green are parental cells that are susceptible to the drug, and the ones in red have an evolved resistance. In the 3 days of the video, you can see the cells growing and expanding. It is the size of these populations that we want to quantify.
In this post, I will remedy last week’s omission and share some empirical population dynamics. As before, I will include some of the Python code I built for these purposes. This time the code is specific to how our microscope exports its data, and so probably not as generalizable as one might want. But hopefully it will still give you some ideas on how to code analysis for your own experiments, dear reader. As always, the code is on my github.
Although the opening video considers two types of cancer cells competing, for the rest of the post I will consider last week’s system: coculturing Alectinib-sensitive (parental) non-small cell lung cancer and fibroblasts in varying concentrations of Alectinib. Finally, this will be another tools post so the only conclusions are of interest as sanity checks. Next week I will move on to more interesting observations using this sort of pipeline.
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